Journal: PLoS ONE

Article Title: A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings

doi: 10.1371/journal.pone.0131701

Figure Lengend Snippet: Fluorescent photos of SYBR-Green based PCR reagents tubes after amplification steps in a commercial cycler and the TTC. The intensity of NTC tube is much lower than the tubes containing template DNA.

Article Snippet: By using extracted Staphylococcus aureus (ATCC 29213) template, PCR reactions targeting the nuc gene (281 bp) were performed using PrimeSTAR Max DNA polymerase master mix in thin-walled PCR tubes (Cat. No. 16950, Sorenson Bioscience Corp, Murray, UT) to amplify the extracted template [ ].

Techniques: SYBR Green Assay, Amplification

Journal: PLoS ONE

Article Title: A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings

doi: 10.1371/journal.pone.0131701

Figure Lengend Snippet: PCR reactions to amplify 281 bp of the nuc gene from 2000, 200, 20, or 2 copies of S . aureus genomic DNA. (A) Lanes 1 to 4: PCR products from commercial thermal cycler. Lane 5: ladder. Lanes 6 to 9: PCR products from TTC with thin-walled plastic tubes, using a protocol of 2 min hot-start, followed by 40 cycles of 11 s and 17 s denaturation and annealing/extension. The 40-cycle reactions were completed in 22 min. (B) Lanes 1 to 4: PCR products from commercial thermal cycler. Lane 5: ladder. Lanes 6 to 9: PCR products from TTC with glass capillary tubes, using a protocol of 2 min hot-start, followed by 40 cycles of 7 s and 8 s denaturation and annealing/extension. The reactions were completed in 13 min and 3 s.

Article Snippet: By using extracted Staphylococcus aureus (ATCC 29213) template, PCR reactions targeting the nuc gene (281 bp) were performed using PrimeSTAR Max DNA polymerase master mix in thin-walled PCR tubes (Cat. No. 16950, Sorenson Bioscience Corp, Murray, UT) to amplify the extracted template [ ].

Techniques: